Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)
![Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)](https://www.abcam.com/ps/products/181/ab181464/Images/ab181464-210815-anti-carbonic-anhydrase-ix-antibody-10f7a8-n-terminal-western-blot.jpg "Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)")
Key features and details
- Mouse monoclonal [10F7A8] to Carbonic Anhydrase 9/CA9 - N-terminal
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal
See all Carbonic Anhydrase 9/CA9 primary antibodies -
Description
Mouse monoclonal [10F7A8] to Carbonic Anhydrase 9/CA9 - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Carbonic Anhydrase 9/CA9 aa 37-186 (N terminal). Expressed in E.coli. Predicted MW of fragment is 42 kDa.
Sequence:PQRL PRMQEDSPLG GGSSGEDDPL GEEDLPSEED SPREEDPPGE EDLPGEEDLP GEEDLPEVKP KSEEEGSLKL EDLPTVEAPG DPQEPQNNAH RDKEGDDQSH WRYGGDPPWP RVSPACAGRF QSPVDIRPQL AAFCPALRPL ELLGFQ
Database link: Q16790 -
Positive control
- Carbonic Anhydrase 9/CA9 recombinant protein, Carbonic Anhydrase IX (amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate, A431 cell line lysate, SW620 cell line lysate, NTERA2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
10F7A8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution
Lane 1 : A431 cell line lysate
Lane 2 : SW620 cell line lysate
Predicted band size: 50 kDa
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![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)](https://www.abcam.com/ps/products/181/ab181464/Images/ab181464-210814-anti-carbonic-anhydrase-ix-antibody-10f7a8-n-terminal-western-blot.jpg)
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution
Lane 1 : HEK293 cell lysate.
Lane 2 : Carbonic Anhydrase 9/CA9 (Amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 50 kDa
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![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)](https://www.abcam.com/ps/products/181/ab181464/Images/ab181464-210813-anti-carbonic-anhydrase-ix-antibody-10f7a8-n-terminal-western-blot.jpg)
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution + Carbonic Anhydrase 9/CA9 recombinant protein
Predicted band size: 50 kDa
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![Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)](https://www.abcam.com/ps/products/181/ab181464/Images/ab181464-210853-anti-carbonic-anhydrase-ix-antibody-10f7a8-n-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)Flow cytometric analysis of NTERA2 cells labeling Carbonic Anhydrase 9/CA9 with ab181464 at 1/200 dilution (green) and negative control (red).
