Anti-RMS1 antibody [EPR20103] (ab202438)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20103] to RMS1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
Overview
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Product name
Anti-RMS1 antibody [EPR20103]
See all RMS1 primary antibodies -
Description
Rabbit monoclonal [EPR20103] to RMS1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saccharomyces cerevisiae whole cell lysate.
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General notes
This product was previously labelled as RKM4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20103 -
Isotype
IgG
Images
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All lanes : Anti-RMS1 antibody [EPR20103] (ab202438) at 1/1000 dilution
Lane 1 : Saccharomyces cerevisiae (wild-type) whole cell lysate
Lane 2 : Saccharomyces cerevisiae (RKM4 KO) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST
RKM4 KO strain (Y03616) was purchased from Euroscarf.
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Anti-RMS1 antibody [EPR20103] (ab202438) at 1/1000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST
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