Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Carbonic Anhydrase 9/CA9 antibody
See all Carbonic Anhydrase 9/CA9 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 9/CA9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Recombinant fragment
-
Immunogen
Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab47011) -
Positive control
- Carbonic Anhydrase 9/CA9 recombinant protein This antibody gave a positive result in IF in the following methanol fixed cell lines: DU145.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB Recombinant fragmentApplication Abreviews Notes WB (2) Use at an assay dependent concentration. Predicted molecular weight: 49.7 kDa.ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use at an assay dependent concentration. Predicted molecular weight: 49.7 kDa.ICC/IF
Use a concentration of 5 µg/ml.Target
-
Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
Tissue specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
Post-translational
modificationsAsn-346 bears high-mannose type glycan structures. -
Cellular localization
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
-
Alternative names
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
Images
-
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471)Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471) at 1 µg/ml + Carbonic Anhydrase IX recombinant protein at 0.1 µg/ml
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49.7 kDa
Observed band size: 53,54 kDa why is the actual band size different from the predicted?This antibody was tested on the Carbonic Anhydrase IX recombinant protein which migrates as a doublet at 53 and 54kDa.
-
Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471)ICC/IF image of ab10471 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10471 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References (0)
ab10471 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471)Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471) at 1 µg/ml + Carbonic Anhydrase IX recombinant protein at 0.1 µg/ml
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49.7 kDa
Observed band size: 53,54 kDa why is the actual band size different from the predicted?This antibody was tested on the Carbonic Anhydrase IX recombinant protein which migrates as a doublet at 53 and 54kDa.
-
Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody (ab10471)
ICC/IF image of ab10471 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10471 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
