Anti-Pirin/PIR antibody (ab21202)
Key features and details
- Goat polyclonal to Pirin/PIR
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pirin/PIR antibody
See all Pirin/PIR primary antibodies -
Description
Goat polyclonal to Pirin/PIR -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human Pirin/PIR (N terminal).
Sequence:GSSKKVTLSVLSREC
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Positive control
- Human heart lysate.
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General notes
Previously labelled as Pirin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab21202)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human spleen staining Pirin/PIR with ab21202 at 5 μg/ml
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Western blot - Anti-Pirin/PIR antibody (ab21202)Anti-Pirin/PIR antibody (ab21202) at 0.3 µg/ml + Human Heart lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 30-35 kDa why is the actual band size different from the predicted?ab21202 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab21202)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human liverstaining Pirin/PIR with ab21202 at 5 μg/ml
