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Anti-Pirin/PIR antibody (ab227280)

Anti-Pirin/PIR antibody (ab227280)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Pirin/PIR
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Pirin/PIR antibody
    See all Pirin/PIR primary antibodies
  • Description

    Rabbit polyclonal to Pirin/PIR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Pirin/PIR (internal sequence). The exact sequence is proprietary.
    Database link: O00625

  • Positive control

    • WB: Mouse liver tissue lysate; HeLa whole cell lysate. IHC-P: Mouse liver tissue; Rat brain stem tissue; MPG xenograft tissue. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as Pirin.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Other Nuclear Bodies
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab227280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab227280)

    Paraffin-embedded MPG xenograft tissue stained for Pirin/PIR using ab227280  at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-Pirin/PIR antibody (ab227280)
    Western blot - Anti-Pirin/PIR antibody (ab227280)
    Anti-Pirin/PIR antibody (ab227280) at 1/1000 dilution + Mouse liver tissue lysate at 50 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Western blot - Anti-Pirin/PIR antibody (ab227280)
    Western blot - Anti-Pirin/PIR antibody (ab227280)
    Anti-Pirin/PIR antibody (ab227280) at 1000 cells + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab227280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab227280)

    Paraffin-embedded mouse liver tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab227280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (ab227280)

    Paraffin-embedded rat brain stem tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pirin/PIR antibody (ab227280)
    Immunocytochemistry/ Immunofluorescence - Anti-Pirin/PIR antibody (ab227280)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Pirin/PIR (green) using ab227280 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-Pirin/PIR antibody [EPR11375(B)] (ab157212)

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    Anti-Pirin/PIR antibody [2740C2] (ab51360)

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