Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR12 antibody [1B8] (ab252878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1B8] to WDR12
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-WDR12 antibody [1B8]
    See all WDR12 primary antibodies

  • Description

    Rat monoclonal [1B8] to WDR12

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human WDR12. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9GZL7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, HEK-293, Jurkat and HepG2 whole cell lysate. ICC/IF: HEK-293 and HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1B8

  • Isotype

    IgG1

  • Light chain type

    lambda

Images

  • Western blot - Anti-WDR12 antibody [1B8] (ab252878)
    Western blot - Anti-WDR12 antibody [1B8] (ab252878)
    All lanes : Anti-WDR12 antibody [1B8] (ab252878) at 1.235 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 48 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1/3: 3 seconds; Lane 2 : 15 seconds; Lane 4: 1 second.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (ab252878)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (ab252878)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling WDR12 with ab252878 at 12.35 µg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (ab252878)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (ab252878)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling WDR12 with ab252878 at 12.35 µg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HEK-293 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-WDR12 antibody [1B8] (ab252878)
    Anti-WDR12 antibody [1B8] (ab252878)

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