Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11944(B)] to CEP55 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free
    See all CEP55 primary antibodies

  • Description

    Rabbit monoclonal [EPR11944(B)] to CEP55 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human CEP55. The exact sequence is proprietary.
    Database link: Q53EZ4

  • General notes

    Ab240159 is the carrier-free version of ab170414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240159 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11944(B)

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)
    Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

    Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)
    Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

    ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

    ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)
    Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

    Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)
    Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

    Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)
    Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

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