Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEP55 antibody [EPR11944(B)] (ab170414)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11944(B)] to CEP55
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CEP55 antibody [EPR11944(B)]
    See all CEP55 primary antibodies

  • Description

    Rabbit monoclonal [EPR11944(B)] to CEP55

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CEP55. The exact sequence is proprietary.
    Database link: Q53EZ4

  • Positive control

    • SW480, HeLa, and HepG2 cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11944(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/5000 dilution + SW480 Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    All lanes : Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

  • Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Anti-CEP55 antibody [EPR11944(B)] (ab170414)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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