Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit recombinant multiclonal [RM1010] to MAP2
Suitable for: ICC, IHC-Fr, Flow Cyt (Intra), WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MAP2 antibody [RM1010]
See all MAP2 primary antibodies
Description

Rabbit recombinant multiclonal [RM1010] to MAP2
Host species

Rabbit
Tested applications

Suitable for: ICC, IHC-Fr, Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SK-N-BE, IMR-32, Neuro-2a and PC-12 whole cell lysate; Mouse E12.5 brain, brain and cerebellum tissue lysates; Rat brain and cerebellum tissue lysates. IHC-Fr: Mouse cerebellum tissue; Rat cerebellum tissue. ICC: Mouse primary neural/glia cells. Flow Cyt: Mouse primary neuron cells; Neuro-2a cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Recombinant Multiclonal
Clone number

RM1010
Isotype

IgG

Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] (ab281588)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry - Anti-MAP2 antibody [RM1010] (ab281588)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with 281588 at 1/2000 (0.276 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry - Anti-MAP2 antibody [RM1010] (ab281588)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Mouse primary neuron cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-MAP2 antibody [RM1010] (ab281588)

All lanes : Anti-MAP2 antibody [RM1010] (ab281588) at 1/1000 dilution

Lane 1 : SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate
Lane 2 : IMR-32 (Human neuroblastoma neuroblast) whole cell lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 199 kDa
Observed band size: 70/280 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.

Exposure time: 48 seconds.
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] (ab281588)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-MAP2 antibody [RM1010] (ab281588)

All lanes : Anti-MAP2 antibody [RM1010] (ab281588) at 1/1000 dilution

Lane 1 : Mouse E12.5 brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse cerebellum lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 199 kDa
Observed band size: 70/280 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.

Exposure time: 3 seconds.
Flow Cytometry - Anti-MAP2 antibody [RM1010] (ab281588)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-MAP2 antibody [RM1010] (ab281588)

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