Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4151(2)] to Carbonic Anhydrase 9/CA9 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free
    See all Carbonic Anhydrase 9/CA9 primary antibodies

  • Description

    Rabbit monoclonal [EPR4151(2)] to Carbonic Anhydrase 9/CA9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 200-300 (extracellular). The exact sequence is proprietary.
    Database link: Q16790

  • Positive control

    • IHC: Human stomach tissue; WB: HT-29 cells and human stomach lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Ab180539 is the carrier-free version of ab108351. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes. 

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab180539 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
    Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
    Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351) at 1/10000 dilution (Purified) + HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 54, 58 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108351, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12966427, 30734594 and 15951534).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
    Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
    Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351) at 1/10000 dilution (Purified) + Human stomach lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 54, 58 kDa why is the actual band size different from the predicted?



    This data was developed using ab108351, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12966427, 30734594 and 15951534).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)

    This data was developed using ab180539, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labeling Carbonic Anhydrase 9/CA9 with Purified ab180539 at 1:150 dilution (0.89 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
    Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)

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