Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [20F8] to LMO 4
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-LMO 4 antibody [20F8]
See all LMO 4 primary antibodies
Description

Rat monoclonal [20F8] to LMO 4
Host species

Rat

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment within Mouse LMO 4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P61969

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Positive control
- WB: Wild-type HAP1 whole cell lysate. Mouse embryo tissue lysate. T47D whole cell lysate. IHC-P: Mouse and rat hippocampus tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Ion Exchange Chromatography
Clonality

Monoclonal
Clone number

20F8
Isotype

IgG2a

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] (ab243139)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling LMO 4 with ab243139 at 1/1000 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat hippocampus tissue is observed. The section was incubated with ab243139 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] (ab243139)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling LMO 4 with ab243139 at 1/1000 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse hippocampus tissue is observed. The section was incubated with ab243139 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Western blot - Anti-LMO 4 antibody [20F8] (ab243139)

All lanes : Anti-LMO 4 antibody [20F8] (ab243139) at 1.354 µg/ml

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 3 : Mouse embryo tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1,2: 15 secs; Lane 3: 3 secs
Anti-LMO 4 antibody [20F8] (ab243139)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com