Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALKBH1 antibody [EPR19215] (ab195376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19215] to ALKBH1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALKBH1 antibody [EPR19215]
    See all ALKBH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19215] to ALKBH1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, K562, A549, A431, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human fetal kidney, colon and fetal liver lysates; mouse brain and spleen lysates; rat spleen lysate. IP: K562 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19215

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ALKBH1 knockout HAP1 whole cell lysate
    Lane 3 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab195376 observed at 43 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab195376 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab195376 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human colon lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Mouse spleen lysate
    Lane 7 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 30 seconds Lane 5-7: 3 minutes.

  • Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (ab195376)
    Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (ab195376)

    ALKBH1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab195376 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab195376 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: K562 whole cell lysate 10µg (Input).

    Lane 2: ab195376 IP in K562 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195376 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-ALKBH1 antibody [EPR19215] (ab195376)
    Anti-ALKBH1 antibody [EPR19215] (ab195376)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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