Anti-PIM1 antibody [ZP003] (ab54503)
![Anti-PIM1 antibody [ZP003] (ab54503)](https://www.abcam.com/ps/products/54/ab54503/Images/ab54503-256179-anti-pim1-antibody-zp003-western-blot.jpg "Anti-PIM1 antibody [ZP003] (ab54503)")
Key features and details
- Mouse monoclonal [ZP003] to PIM1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-PIM1 antibody [ZP003]
See all PIM1 primary antibodies -
Description
Mouse monoclonal [ZP003] to PIM1 -
Host species
Mouse -
Specificity
ab54503 is specific for the PIM1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide derived from the internal region of the human PIM1 protein, which is 94% and 88% homologous with rat and mouse, respectively.
-
Positive control
- WB: K562 cytoplasmic or nuclear lysate.
Properties
-
Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
ZP003 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-PIM1 antibody [ZP003] (ab54503)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PIM1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab54503 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab54503 was shown to recognize PIM1 when PIM1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PIM1 knockout samples were subjected to SDS-PAGE. ab54503 and ab181602 (loading control to GAPDH) were diluted 3 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-PIM1 antibody [ZP003] (ab54503)](https://www.abcam.com/ps/products/54/ab54503/Images/ab54503-67384-anti-pim1-antibody-zp003-western-blot.jpg)
Western blot - Anti-PIM1 antibody [ZP003] (ab54503)Anti-PIM1 antibody [ZP003] (ab54503) at 3 µg/ml + K562 cell lysate
Observed band size: 35 kDa why is the actual band size different from the predicted?
