Anti-PIM1 (phospho Y309) antibody (ab74047)
Key features and details
- Rabbit polyclonal to PIM1 (phospho Y309)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIM1 (phospho Y309) antibody
See all PIM1 primary antibodies -
Description
Rabbit polyclonal to PIM1 (phospho Y309) -
Host species
Rabbit -
Specificity
ab74047 detects endogenous levels of human PIM1 only when phosphorylated at tyrosine 309. ab74047 detects endogenous levels of mouse PIM1 only when phosphorylated at tyrosine 302 and rat PIM1 only when phosphorylated at tyrosine 218. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).
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Positive control
- HUVEC cell extracts treated with PMA (125ng/ml, 30mins).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab74047 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution
Lane 1 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins)
Lane 2 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa