Native Human Fibronectin protein (ab80021)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Native Human Fibronectin protein
See all Fibronectin proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purified from human plasma -
Expression system
Native -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Sequence
QAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGG SRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRG RISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKP IAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRC NDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSS GSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQM LCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQD GHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNY TDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIG DQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTF HKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVH GVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWN APQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQL ISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEI TASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLL PGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRW SRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIY AVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPE SAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVS HGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTV GLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGV FTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREV TSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLH LEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSS CTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNIGPD TMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPG TEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAP RATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVAL NGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRIT YGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASS KPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNG PGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTN IDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPD GEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFT QVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSG LMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTIT ISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKI YLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARIT GYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQK SEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTG NGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVG EEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSE YIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRH KVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKL LCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGN GKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCD NCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADR EDSRE -
Amino acids
32 to 2386 -
Additional sequence information
Molecular weight: the MW of the hetrodimer is 550 kDa
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.476% HEPES, 0.87% Sodium chloride -
ReconstitutionReconstitute with 1.0 milliliters of water to achieve 2.0 mg/mL. Human Fibronectin is known for its solubility issues. The best way to get this material into solution, after reconstitution, is to incubate in a 37ºC water bath for at least 30 minutes. Typically this is sufficient, however a small amount of un-dissolved material may remain. If this is the case, a few gentle aspirations with a pipette is recommended.
Images
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SDS-PAGE - Native Human Fibronectin protein (ab80021)
Gel: Novex 4-12% Bis-Tris
Load Load: Human Fibronectin, 1 µg per lane in Non-reducing (NR) and reducing (R) conditions.
Buffer: MOPS
MW Ladder: Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)
