Anti-CD4 antibody (ab203034)
Key features and details
- Rabbit polyclonal to CD4
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-CD4 antibody
See all CD4 primary antibodies -
Description
Rabbit polyclonal to CD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment
Predicted to work with: Pig
Does not react with:
Mouse -
Immunogen
Synthetic peptide within Rat CD4 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P05540 -
Positive control
- IHC-P: Human colon carcinoma, rat kidney, human kidney and rat lung tissues. WB: CD4 recombinant protein; Raji cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD4 antibody (ab203034)Anti-CD4 antibody (ab203034) at 1/300 dilution + U937 Cell Lysate at 40 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody (ab203034)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling CD4 with ab203034 at 1/200 dilution overnight, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C.
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Western blot - Anti-CD4 antibody (ab203034)Anti-CD4 antibody (ab203034) at 1/300 dilution + HL60 Cell Lysate at 40 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody (ab203034)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat kidney tissue labeling CD4 with ab203034 at 1/400 dilution overnight at 4°C followed by a conjugated secondary for 20 minutes and DAB staining.
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Western blot - Anti-CD4 antibody (ab203034)All lanes : Anti-CD4 antibody (ab203034) at 1/300 dilution
Lane 1 : CD4 recombinant protein
Lane 2 : Raji cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 51 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody (ab203034)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling CD4 with ab203034 at 1/400 dilution followed by conjugation to the secondary antibody and DAB staining.
