Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22065] to CD7
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-CD7 antibody [EPR22065]
See all CD7 primary antibodies
Description

Rabbit monoclonal [EPR22065] to CD7
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MOLT-4 and HepG2 whole cell lysates; Human tonsil and thymus lysates. IP: Human tonsil lysate. ICC/IF: MOLT-4 cells. Flow cyt: Human PBMCs.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22065
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD7 antibody [EPR22065] (ab224194)

Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor^® 647-conjugated anti-human CD3 and rabbit IgG - ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight^® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID: 3260936)
Western blot - Anti-CD7 antibody [EPR22065] (ab224194)

All lanes : Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

Lane 1 : Human tonsil lysate
Lane 2 : Human thymus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times:

Lane 1: 3 minutes

Lane 2: 48 seconds

This blot was developed using a higher sensitivity ECL substrate.

The molecular mass observed is consistent with what has been described in the literature (PMID: 24157461, PMID: 2479685); the actual size different from the predicted is due to glycosylation (PMID: 2479685).
Western blot - Anti-CD7 antibody [EPR22065] (ab224194)

All lanes : Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 25 kDa
Additional bands at: 37 kDa (possible non-specific secondary antibody binding)

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

The molecular mass observed is consistent with what has been described in the literature (PMID: 24157461, PMID: 2479685); the actual size different from the predicted is due to glycosylation (PMID: 2479685).

Negative control: Raji (PMID: 1633296, PMID: 11389079).
Immunoprecipitation - Anti-CD7 antibody [EPR22065] (ab224194)

CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Human tonsil lysate 10 μg (Input).
Lane 2: ab224194 IP in human tonsil lysate lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224194 in human tonsil lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (ab224194)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

PDS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control: Raji (PMID: 16332967).
Anti-CD7 antibody [EPR22065] (ab224194)

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