Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19550] to SAA1 + SAA2
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-SAA1 + SAA2 antibody [EPR19550]
See all SAA1 + SAA2 primary antibodies -
Description
Rabbit monoclonal [EPR19550] to SAA1 + SAA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanRecombinant fragment -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human SAA1 active recombinant protein; human SAA2 fragment recombinant protein; human serum; human liver lysate. IHC-P: Human liver and liver cancer tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19550 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human liver is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/1000 dilution
Lane 1 : Human SAA1 active recombinant protein
Lane 2 : Human SAA2 fragment recombinant protein
Lane 3 : Human SAA4 fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 13,35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Human SAA2 and SAA4 fragment recombinant protein contain aa19-122 and aa19-130 with His-Tag® and GST-tag.
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Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/5000 dilution + Human serum at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Expressed by the liver; secreted in plasma and serum (PMID: 3183061; PMID: 16039662).
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Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/1000 dilution + Human liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Expressed by the liver; secreted in plasma and serum (PMID: 3183061; PMID: 16039662).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human liver cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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