Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

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Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19235] to SAA1 + SAA2
Suitable for: IHC-Fr, IP, IHC-P, WB
Reacts with: Mouse

Product name

Anti-SAA1 + SAA2 antibody [EPR19235]
See all SAA1 + SAA2 primary antibodies
Description

Rabbit monoclonal [EPR19235] to SAA1 + SAA2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant mouse SAA1 protein (aa20-122); GST and His-tagged recombinant mouse SAA2 protein (aa20-122); Mouse plasma and serum. IHC-P: Mouse liver tissue. IHC-Fr: Mouse liver tissue. IP: Mouse serum.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19235
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse kidney.

Negative control: mouse kidney (PMID:19747761; PMID: 15623807).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/100 dilution (green). Cytoplasmic staining on mouse liver is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

All lanes : Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/10000 dilution

Lane 1 : Recombinant mouse SAA1 protein (aa20-122) at 0.01 µg
Lane 2 : GST and His-tagged recombinant mouse SAA2 protein (aa20-122) at 0.005 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa
Observed band size: 13 kDa

Exposure time: 2 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/2000 dilution + GST and His-tagged recombinant mouse SAA3 protein (aa1-122) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

All lanes : Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/1000 dilution

Lane 1 : Mouse plasma
Lane 2 : Mouse serum

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa
Observed band size: 13 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.
Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

SAA1 + SAA2 was immunoprecipitated from 1 mg of mouse serum with ab199030 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199030 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse serum 10 μg (Input).

Lane 2: ab199030 IP in mouse serum.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199030 in mouse serum.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

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