Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Selenium Binding Protein 1/SBP antibody (ab72249)

Key features and details

  • Rabbit polyclonal to Selenium Binding Protein 1/SBP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Selenium Binding Protein 1/SBP antibody
    See all Selenium Binding Protein 1/SBP primary antibodies

  • Description

    Rabbit polyclonal to Selenium Binding Protein 1/SBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Xenopus laevis, Non human primates

  • Immunogen

    Synthetic peptide corresponding to Human Selenium Binding Protein 1/SBP aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90221)

  • Positive control

    • This antibody gave a positive signal in the following human tissue lysates: Liver; Colon; Spleen.

  • General notes

    Previously labelled as Selenium Binding Protein 1. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab72249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.

  • Tissue specificity

    Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.

  • Sequence similarities

    Belongs to the selenium-binding protein family.

  • Post-translational
    modifications

    Phosphorylated.
    The N-terminus is blocked.

  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Membrane. May associate with Golgi membrane. May associate with the membrane of autophagosomes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 56 kDa selenium binding protein antibody
    • 56 kDa selenium-binding protein antibody
    • hSBP antibody
    • LPSB antibody
    • SBP antibody
    • SBP1 antibody
    • SBP1_HUMAN antibody
    • SBP56 antibody
    • Selenbp1 antibody
    • Selenbp2 antibody
    • Selenium Binding Protein 1 antibody
    • Selenium binding protein 2 antibody
    • Selenium binding protein1 antibody
    • Selenium-binding protein 1 antibody
    • SELNBP1 antibody
    • SP56 antibody
    see all

Images

  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab72249) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    ICC/IF image of ab72249 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72249, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.

References (1)

Publishing research using ab72249? Please let us know so that we can cite the reference in this datasheet.

ab72249 has been referenced in 1 publication.

  • Gupta S  et al. Evaluation of autoantibody signatures in meningioma patients using human proteome arrays. Oncotarget 8:58443-58456 (2017). PubMed: 28938569

Images

  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab72249) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab72249)
    ICC/IF image of ab72249 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72249, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.

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