PE Anti-Cortactin antibody [EP1922Y] (ab209758)
![PE Anti-Cortactin antibody [EP1922Y] (ab209758)](https://www.abcam.com/ps/products/209/ab209758/Images/ab209758-265195-anti-cortactin-antibody-ep1922y-phycoerythrin-flow-cytometry.jpg "PE Anti-Cortactin antibody [EP1922Y] (ab209758)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1922Y] to Cortactin
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Cortactin antibody [EP1922Y]
See all Cortactin primary antibodies -
Description
PE Rabbit monoclonal [EP1922Y] to Cortactin -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1922Y -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - PE Anti-Cortactin antibody [EP1922Y] (ab209758)
Overlay histogram showing HeLa cells stained with ab209758 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209758, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions. -
![PE Anti-Cortactin antibody [EP1922Y] (ab209758)](https://www.abcam.com/ps/products/209/ab209758/Images/ab209758-3-benefits-of-recombinant-antibodies.png)
PE Anti-Cortactin antibody [EP1922Y] (ab209758)
