Anti-DDX3 antibody [6G8-F4-E3] (ab196032)
![Anti-DDX3 antibody [6G8-F4-E3] (ab196032)](https://www.abcam.com/ps/products/196/ab196032/Images/ab196032-316812-anti-ddx3-antibody-6g8-f4-e3-western-blot.jpg "Anti-DDX3 antibody [6G8-F4-E3] (ab196032)")
Key features and details
- Mouse monoclonal [6G8-F4-E3] to DDX3
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-DDX3 antibody [6G8-F4-E3]
See all DDX3 primary antibodies -
Description
Mouse monoclonal [6G8-F4-E3] to DDX3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB MouseRatHumanAfrican green monkey
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Immunogen
Recombinant fragment corresponding to Human DDX3. (Purified fragments expressed in E.coli; NM_001356).
Database link: O00571 -
Positive control
- Jurkat, K562,COS7, C6, 3T3 and HeLa whole cell lysate (ab150035); HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
6G8-F4-E3 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)All lanes : Anti-DDX3 antibody [6G8-F4-E3] (ab196032) at 1/1000 dilution
Lane 1 : COS7 Cell lysates
Lane 2 : Jurkat Cell lysates
Lane 3 : C6 Cell lysates
Lane 4 : 3T3 Cell lysates
Lane 5 : HeLa Cell lysates
Predicted band size: 73 kDa
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![Western blot - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)](https://www.abcam.com/ps/products/196/ab196032/Images/ab196032-233367-anti-ddx3-antibody-6g8-f4-e3-western-blot.jpg)
Western blot - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)All lanes : Anti-DDX3 antibody [6G8-F4-E3] (ab196032) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : 3T3 cell lysate
Lane 6 : HeLa cell lysate
Predicted band size: 73 kDaThis image was produced with antibody produced in ascites.
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![Immunocytochemistry/ Immunofluorescence - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)](https://www.abcam.com/ps/products/196/ab196032/Images/ab196032-233368-anti-ddx3-antibody-6g8-f4-e3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)Immunofluorescent analysis of 4% parafomaldehyde-fixed HeLa cells labeling DDX3 with ab196032 at 1/200 dilution. This image was produced with antibody produced in ascites.
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![Immunoprecipitation - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)](https://www.abcam.com/ps/products/196/ab196032/Images/ab196032-233369-anti-ddx3-antibody-6g8-f4-e3-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DDX3 antibody [6G8-F4-E3] (ab196032)Detection of DDX3 in immunoprecipitates of HeLa cell lysate immunoprecipitated using ab196032 (Lane 2). Lane 1 represents control IgG IP. This image was produced with antibody produced in ascites.
