Anti-CELSR1 antibody (ab225889)
Key features and details
- Rabbit polyclonal to CELSR1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CELSR1 antibody
See all CELSR1 primary antibodies -
Description
Rabbit polyclonal to CELSR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human CELSR1 aa 2775-2825. The exact sequence is proprietary. (NP_055061.1).
Database link: Q9NYQ6 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225889 was affinity purified using an epitope specific to TRAF2 immobilized on solid support. -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-CELSR1 antibody (ab225889)All lanes : Anti-CELSR1 antibody (ab225889) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 329 kDa
Exposure time: 3 minutesLysates prepared using NETN buffer.
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Immunoprecipitation - Anti-CELSR1 antibody (ab225889)CELSR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225889 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225889 at 0.4 µg/ml.
Lane 1: ab225889 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
