Zinc Assay Kit (Cell-based) (ab241014)
Key features and details
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Zinc Assay Kit (Cell-based)
See all Zinc kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Product overview
Zinc Assay Kit (Cell-based) (ab241014) allows studies of the regulation of Zinc at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.
This kit contains a selective and membrane permeable fluorescent probe for detection of intracellular Zinc. We also include a Positive Control that elevates Zinc staining, and cell- permeable Zinc Chelator that reduces fluorescence induced by Positive Control.
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Tested applications
Suitable for: FM, Flow Cytmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 50 tests Assay Buffer (10X) 1 x 25ml Positive Control (2000X) 1 x 100µl Zinc Chelator (500X) 1 x 100µl Zinc Probe (500X) 1 x 1ml -
Research areas
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Relevance
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. -
Alternative names
- Zn
- Zn++
- Zn2+
Images
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Zinc Staining in Jurkat and HeLa cells.
1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control, or Positive Control plus Zinc Chelator for 30 minutes followed by Zinc Staining according to kit protocol. Histograms from flow analysis of untreated cells stained with Zinc Probe (Green Line) and cells treated with Positive Control (Pink Line) show increased signal in cells treated with Positive control reagent compared to untreated cells.
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Zinc Staining in Jurkat and HeLa cells.1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control, or Positive Control plus Zinc Chelator for 30 minutes followed by Zinc Staining according to kits protocol. Positive Control Treated Cells without (Pink Line) and with Zinc Chelator treated Cells (Blue Line). Decreased fluorescence in presence of Zinc Chelator confirms that the fluorescence is generated by intracellular Zinc.
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Zinc Staining in Jurkat and HeLa cells.Fluorescence microscope images of Basal Zinc Staining (Green) and Positive Reagent treated cells. -
Zinc Staining in Jurkat and HeLa cells.Increased Green fluorescence induced by treatment with Positive Control compared to the Basal Zinc levels confirms intracellular accumulation of fluorescent stain in the cells.
