Anti-nNOS (neuronal) antibody (ab1376)
Key features and details
- Goat polyclonal to nNOS (neuronal)
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-nNOS (neuronal) antibody
See all nNOS (neuronal) primary antibodies -
Description
Goat polyclonal to nNOS (neuronal) -
Host species
Goat -
Tested applications
Suitable for: ICC, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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Positive control
- ICC: HeLa cells. Flow Cyt (intra): KELLY cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab1376)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Incuabted with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml).
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Flow Cytometry - Anti-nNOS (neuronal) antibody (ab1376)Flow cytometric analysis of paraformaldehyde fixed KELLY (Human neuroblastoma cell line) cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
