Anti-nNOS (neuronal) antibody (ab40662)
Key features and details
- Rabbit polyclonal to nNOS (neuronal)
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-nNOS (neuronal) antibody
See all nNOS (neuronal) primary antibodies -
Description
Rabbit polyclonal to nNOS (neuronal) -
Host species
Rabbit -
Specificity
This antiserum is specific for the carboxyl end of hnNOS. Reactivity: hnNOS(1411-1433) 100%; recombinant hnNOS (NOS type I) 100% Does not cross react with hiNOS(1137-1153), recombinant hiNOS (NOS type II), heNOS(1182-1203) or recombinant heNOS (type III) -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Rat
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Immunogen
Synthetic peptide:
NRLRSESIAFIEESKKDTDEVFS
conjugated to KLH, corresponding to C terminal amino acids 1411-1433 of Human nNOS (neuronal)
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab40662)ICC/IF image of ab40662 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40662, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
