Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)
![Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)](https://www.abcam.com/ps/products/251/ab251432/Images/ab251432-403396-anti-inos-antibody-epr16630-bsa-and-azide-free-western-blot.jpg "Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16630] to iNOS - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-iNOS antibody [EPR16630] - BSA and Azide free
See all iNOS primary antibodies -
Description
Rabbit monoclonal [EPR16630] to iNOS - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251432 is the carrier-free version of ab205529. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251432 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16630 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)All lanes : Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 µg/ml LPS for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 1 secondThis data was developed using ab205529, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)](https://www.abcam.com/ps/products/251/ab251432/Images/ab251432-1-anti-inos-antibody-epr16630-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)This data was developed using ab205529, the same antibody clone in a different buffer formulation.iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell extract with ab205529 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab205529 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract 10ug (Input). Lane 2: ab205529 IP in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205529 in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 8 seconds. -
![Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)](https://www.abcam.com/ps/products/251/ab251432/Images/ab251432-3-benefits-of-recombinant-antibodies.png)
Anti-iNOS antibody [EPR16630] - BSA and Azide free (ab251432)
