Anti-DDAH2 antibody (ab232694)
Key features and details
- Rabbit polyclonal to DDAH2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-DDAH2 antibody
See all DDAH2 primary antibodies -
Description
Rabbit polyclonal to DDAH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment (His-tag) corresponding to Human DDAH2 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: O95865 -
Positive control
- WB: Recombinant human DDAH2 protein; Pig brain lysate; MCF7 cell lysate. IHC-P: Human prostate, brain, colorectal cancer, glioma and stomach cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232694 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab232694)
Paraffin-embedded human prostate tissue stained for DDAH2 using ab232694 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab232694)Paraffin-embedded human brain tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-DDAH2 antibody (ab232694)Anti-DDAH2 antibody (ab232694) at 2 µg/ml + Recombinant human DDAH2 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa
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Western blot - Anti-DDAH2 antibody (ab232694)All lanes : Anti-DDAH2 antibody (ab232694) at 2 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab232694)Paraffin-embedded human colorectal cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab232694)Paraffin-embedded human glioma tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab232694)Paraffin-embedded human stomach cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
