Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20851] to SEH1L - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SEH1L antibody [EPR20851] - BSA and Azide free
    See all SEH1L primary antibodies

  • Description

    Rabbit monoclonal [EPR20851] to SEH1L - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T whole cell lysate.

  • General notes

    Ab235840 is the carrier-free version of ab218531. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab235840 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)
    Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)

    SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HEK-293T whole cell lysate 10 µg (Input).
    Lane 2: ab218531 IP in HEK-293T whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218531 in HEK-293T whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315).

    According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

  • Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)
    Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)
    All lanes : Anti-SEH1L antibody [EPR20851] (ab218531) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.

    The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

     

  • Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)
    Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)

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