Anti-SEH1L antibody [EPR20851] (ab218531)
![Anti-SEH1L antibody [EPR20851] (ab218531)](https://www.abcam.com/ps/products/218/ab218531/Images/ab218531-311112-anti-seh1l-antibody-epr20851-immunoprecipitation.jpg "Anti-SEH1L antibody [EPR20851] (ab218531)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20851] to SEH1L
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SEH1L antibody [EPR20851]
See all SEH1L primary antibodies -
Description
Rabbit monoclonal [EPR20851] to SEH1L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T whole cell lysate. Mouse liver lysate. IP: HEK-293T whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20851 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (ab218531)
SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: ab218531 IP in HEK-293T whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218531 in HEK-293T whole cell lysate (-).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315).
According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.
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![Western blot - Anti-SEH1L antibody [EPR20851] (ab218531)](https://www.abcam.com/ps/products/218/ab218531/Images/ab218531-311110-anti-seh1l-antibody-epr20851-western-blot.jpg)
Western blot - Anti-SEH1L antibody [EPR20851] (ab218531)All lanes : Anti-SEH1L antibody [EPR20851] (ab218531) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.
The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.
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![Western blot - Anti-SEH1L antibody [EPR20851] (ab218531)](https://www.abcam.com/ps/products/218/ab218531/Images/ab218531-311111-anti-seh1l-antibody-epr20851-western-blot.jpg)
Western blot - Anti-SEH1L antibody [EPR20851] (ab218531)Anti-SEH1L antibody [EPR20851] (ab218531) at 1/1000 dilution + Mouse liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315).
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![Anti-SEH1L antibody [EPR20851] (ab218531)](https://www.abcam.com/ps/products/218/ab218531/Images/ab218531-4-benefits-of-recombinant-antibodies.png)
Anti-SEH1L antibody [EPR20851] (ab218531)
