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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Metabolism Vitamins / Minerals

Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)

Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9660] to CBR1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-CBR1 antibody [EPR9660] - BSA and Azide free
    See all CBR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9660] to CBR1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549 and HeLa cell lysates.
  • General notes

    ab249278 is the carrier-free version of ab156590.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9660
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Images

  • Western blot - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Western blot - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 30 kDa



    This data was developed using ab156590, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    This data was developed using ab156590, the same antibody clone in a different buffer formulation.ab156590 showing +ve staining in Human thyroid gland carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    This data was developed using ab156590, the same antibody clone in a different buffer formulation.ab156590 showing +ve staining in Human skeletal muscle. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    This data was developed using ab156590, the same antibody clone in a different buffer formulation.ab156590 showing +ve staining in Human gastric carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    This data was developed using ab156590, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CBR1 with ab156590 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Immunocytochemistry - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    This data was developed using ab156590, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling CBR1 with ab156590 at 1/50 dilution.
  • Western blot - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Western blot - Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : CBR1 knockout A549 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab156590).

    Lanes 1-3: Merged signal (red and green). Green - ab156590 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab156590 Anti-CBR1 antibody [EPR9660] was shown to specifically react with CBR1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266954 (knockout cell lysate ab257874) was used. Wild-type and CBR1 knockout samples were subjected to SDS-PAGE. ab156590 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)
    Anti-CBR1 antibody [EPR9660] - BSA and Azide free (ab249278)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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