Anti-RFC antibody (ab272354)
Key features and details
- Rabbit polyclonal to RFC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-RFC antibody -
Description
Rabbit polyclonal to RFC -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanAfrican green monkey
-
Immunogen
Synthetic peptide corresponding to Human RFC aa 200-300.
Database link: P41440 -
Positive control
- WB: Rat brain and ovary tissue lysates; mouse brain, testis and ovary lysates; human placenta tissue lysate; HEK-293, PC-3 , 22RV1, K562, NIH/3T3 and COS-7 whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate dibasic dihydrate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-RFC antibody (ab272354)All lanes : Anti-RFC antibody (ab272354) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysates
Lane 2 : Rat ovary tissue lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Mouse testicular tissue lysates
Lane 5 : Mouse ovary tissue lysates
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates
Lane 7 : Human placenta tissue lysates
Lane 8 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 10 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysates
Lane 11 : 22RV1 (human prostate carcinoma cell line) whole cell lysates
Lane 12 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa5-20% SDS-PAGE gel.
Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.
