Human AMN ELISA kit (ab277417)
Key features and details
- Sensitivity: 21 pg/ml
- Range: 20.5 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human AMN ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
21 pg/ml -
Range
20.5 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 116.6 87% - 145% Plasma 75.71 70% - 89% Cell culture media 125.4 92% - 147% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human AMN ELISA Kit (ab277417) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human AMN in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended), and cell culture supernatants.
This assay employs an antibody specific for Human AMN coated on a 96-well plate. Standards and samples are pipetted into the wells and Human AMN present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human AMN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human AMN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human AMN Antibody 2 vials Anti-Human AMN coated Microplate 1 unit Human AMN standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm. -
Tissue specificity
Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and peripheral blood leukocytes. -
Involvement in disease
Defects in AMN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1) [MIM:261100]; also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. -
Sequence similarities
Contains 1 VWFC domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 5033428N14Rik
- amn
- Amnion associated transmembrane protein
see all -
Database links
- Entrez Gene: 81693 Human
- Omim: 605799 Human
- SwissProt: Q9BXJ7 Human
- Unigene: 534494 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.