Fumarate Assay Kit (ab102516)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 nmol/well
Overview
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Product name
Fumarate Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 1 nmol/well -
Assay time
0h 40m -
Product overview
Fumarate Assay Kit ab102516 provides a convenient tool for sensitive detection of Fumarate in biological samples.
In the fumarate assay protocol, the fumarate enzyme mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).
Fumurate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 1 hr
- analyze with a microplate reader -
Notes
Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Fumarate Assay Buffer WM 1 x 25ml Fumarate Developer Red 1 vial Fumarate Enzyme Mix Green 1 vial Fumarate Standard Yellow 1 x 0.2ml -
Research areas
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Relevance
Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy.
Images
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Functional Studies - Fumarate Detection Kit (ab102516)Fumurate Standard Curve
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Functional Studies - Fumarate Detection Kit (ab102516)Fumarate measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
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Functional Studies - Fumarate Detection Kit (ab102516)Fumarate measured in biological fluids, background signal subtracted (duplicates +/- SD).
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Functional Studies - Fumarate Detection Kit (ab102516)Fumarate measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
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Functional Studies - Fumarate Detection Kit (ab102516)Fumarate measured in cell lysates, background signal subtracted (duplicates +/- SD).