Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048)
Key features and details
- Rabbit polyclonal to Cytochrome b reductase 1/DCYTB
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome b reductase 1/DCYTB antibody
See all Cytochrome b reductase 1/DCYTB primary antibodies -
Description
Rabbit polyclonal to Cytochrome b reductase 1/DCYTB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF (1) Use a concentration of 10 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Notes ICC/IF
Use a concentration of 10 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Target
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Function
Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells. -
Tissue specificity
Present in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium. -
Sequence similarities
Contains 1 cytochrome b561 domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 79901 Human
- Entrez Gene: 73649 Mouse
- Entrez Gene: 295669 Rat
- Omim: 605745 Human
- SwissProt: Q53TN4 Human
- SwissProt: Q925G2 Mouse
- SwissProt: Q5RKJ2 Rat
- Unigene: 726027 Human
see all -
Alternative names
- CYBR 1 antibody
- CYBR1 antibody
- CYBR1_HUMAN antibody
see all
Images
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Western blot - Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048)Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048) at 1 µg/ml +
A20 whole cell lysate (ab7180) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 10 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048)ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab66048 has been referenced in 3 publications.
- Ma J et al. miR-423-3p activates FAK signaling pathway to drive EMT process and tumor growth in lung adenocarcinoma through targeting CYBRD1. J Clin Lab Anal 35:e24044 (2021). PubMed: 34714955
- Fang W et al. Molecular Hydrogen Protects Human Melanocytes from Oxidative Stress by Activating Nrf2 Signaling. J Invest Dermatol 140:2230-2241.e9 (2020). PubMed: 32234461
- Sherman HG et al. Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. Biochim Biophys Acta Bioenerg 1860:628-639 (2019). PubMed: 31229569
Images
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Western blot - Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048)Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048) at 1 µg/ml +
A20 whole cell lysate (ab7180) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 10 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048)ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
