Anti-nNOS (neuronal) antibody (ab95436)
Key features and details
- Rabbit polyclonal to nNOS (neuronal)
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-nNOS (neuronal) antibody
See all nNOS (neuronal) primary antibodies -
Description
Rabbit polyclonal to nNOS (neuronal) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Lizard -
Immunogen
Synthetic peptide:
IAFIEESKKDTDEVFSS
, corresponding to C terminal amino acids 1418-1434 of Human nNOS (neuronal) -
Positive control
- WB: Whole cell lysate from MCF7, SMMC, SW620 and COLO 320 cells, Rat brain tissue lysate. IHC-P: Rat brain tissue. Rat medulla oblongata tissue.
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General notes
The immunogen sequence is identical to the related rat sequenceReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-nNOS (neuronal) antibody (ab95436) at 0.5 µg/ml
Lane 1 : Rat Brain Tissue Lysate at 50 µg
Lane 2 : MCF-7 Whole Cell Lysate at 40 µg
Predicted band size: 161 kDa
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Immunohistochemistry of rat brain tissue staining nNOS (neuronal).
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ab95436 at 2µg/ml staining NOS in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed.
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All lanes : Anti-nNOS (neuronal) antibody (ab95436) at 1 µg/ml
Lanes 1-2 : Lysate prepared from rat brain tissue.
Lane 3 : Lysate prepared from rat medulla oblongata tissue.
Lane 4 : Whole cell lysate prepared from MCF7 cells.
Lane 5 : Whole cell lysate prepared from SMMC cells.
Lane 6 : Whole cell lysate prepared from SW620 cells.
Lane 7 : Whole cell lysate prepared from Colo320 cells.
Predicted band size: 161 kDa