Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17539-95] to Clusterin alpha chain
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Clusterin alpha chain antibody [EPR17539-95]
    See all Clusterin alpha chain primary antibodies

  • Description

    Rabbit monoclonal [EPR17539-95] to Clusterin alpha chain

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum, lung and adrenal gland lysate; rat serum lysate. IHC-P: Mouse and spleen and adrenal gland tissue; rat spleen and adrenal gland tissue. IHC-Fr: Mouse testis and brain (lateral ventricle) tissue; rat testis and brain (lateral ventricle) tissue. IP: Mouse serum.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    All lanes : Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100) at 1/1000 dilution

    Lane 1 : Mouse serum lysate at 20 µg
    Lane 2 : Rat serum lysate at 10 µg
    Lane 3 : Mouse lung lysate at 10 µg
    Lane 4 : Mouse adrenal gland lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure times:

    Lanes 1,3,4: 24 seconds;

    Lane 2: 3 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 8373947).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of rat adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of mouse adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of frozen rat brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in rat brain (PMID:18620027; 21385939) is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells (PMID: 22552734) is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in mouse brain (PMID:18620027; 21385939) is observed. 

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Immunohistochemical analysis of 4% paraformaldehude-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells is observed; also observe particles deposited on sperm membrane (PMID: 22552734).

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

    Clusterin alpha chain was immunoprecipitated from 0.35 mg mouse serum lysate with ab184100 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184100 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse serum lysate 10 µl (Input).
    Lane 2: ab184100 IP in mouse serum lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184100 in mouse serum lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
    Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

© 2021 Astana Biomed Group LLP. All rights reserved.