Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 10 µl 117 264 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17539-95] to Clusterin alpha chain
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat

Product name

Anti-Clusterin alpha chain antibody [EPR17539-95]
See all Clusterin alpha chain primary antibodies
Description

Rabbit monoclonal [EPR17539-95] to Clusterin alpha chain
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse serum, lung and adrenal gland lysate; rat serum lysate. IHC-P: Mouse and spleen and adrenal gland tissue; rat spleen and adrenal gland tissue. IHC-Fr: Mouse testis and brain (lateral ventricle) tissue; rat testis and brain (lateral ventricle) tissue. IP: Mouse serum.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Isotype

IgG
Research areas

Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

All lanes : Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100) at 1/1000 dilution

Lane 1 : Mouse serum lysate at 20 µg
Lane 2 : Rat serum lysate at 10 µg
Lane 3 : Mouse lung lysate at 10 µg
Lane 4 : Mouse adrenal gland lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

Exposure times:

Lanes 1,3,4: 24 seconds;

Lane 2: 3 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 8373947).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of rat adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of mouse adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of frozen rat brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in rat brain (PMID:18620027; 21385939) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells (PMID: 22552734) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in mouse brain (PMID:18620027; 21385939) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Immunohistochemical analysis of 4% paraformaldehude-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells is observed; also observe particles deposited on sperm membrane (PMID: 22552734).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Clusterin alpha chain was immunoprecipitated from 0.35 mg mouse serum lysate with ab184100 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184100 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Mouse serum lysate 10 µl (Input).
Lane 2: ab184100 IP in mouse serum lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184100 in mouse serum lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

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