Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)
Key features and details
- Mouse monoclonal [OTI1A2] to Tristetraprolin/TTP
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Dog, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Tristetraprolin/TTP antibody [OTI1A2]
See all Tristetraprolin/TTP primary antibodies -
Description
Mouse monoclonal [OTI1A2] to Tristetraprolin/TTP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseHumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human Tristetraprolin/TTP aa 1 to the C-terminus. Produced in HEK-293T cells.
Database link: NP_003398 -
Positive control
- WB: SVT2, A549 and MDCK cell extracts; Mouse liver extract; HEK-293T cell lysate transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA. Flow Cyt: HeLa and Jurkat cells. IHC-P: Human breast, lung carcinoma, pancreas carcinoma, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues.
-
General notes
Clone OTI1A2 (formerly 1A2).
This product was previously labelled as Tristetraprolin
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1A2 -
Isotype
IgG2a -
Research areas
Images
-
All lanes : Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779) at 1/200 dilution
Lane 1 : SVT2 cell extract
Lane 2 : A549 (human lung carcinoma cell line) cell extract
Lane 3 : MDCK (canine kidney cell line) cell extract
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
-
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Tristetraprolin/TTP with ab119779 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
-
Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779) at 1/200 dilution + Mouse liver extract at 10 µg
Predicted band size: 34 kDa
-
All lanes : Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 34 kDa
-
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling Tristetraprolin/TTP with ab119779 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
-
Paraffin-embedded human bladder carcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human breast tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human endometrium tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human endometrium adenocarcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human lung carcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human pancreas carcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human tonsil tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.