Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Key features and details

Mouse monoclonal [OTI1A2] to Tristetraprolin/TTP
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Dog, Human
Isotype: IgG2a

Product name

Anti-Tristetraprolin/TTP antibody [OTI1A2]
See all Tristetraprolin/TTP primary antibodies
Description

Mouse monoclonal [OTI1A2] to Tristetraprolin/TTP
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Tristetraprolin/TTP aa 1 to the C-terminus. Produced in HEK-293T cells.
Database link: NP_003398

Run BLAST with Run BLAST with
Positive control
- WB: SVT2, A549 and MDCK cell extracts; Mouse liver extract; HEK-293T cell lysate transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA. Flow Cyt: HeLa and Jurkat cells. IHC-P: Human breast, lung carcinoma, pancreas carcinoma, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues.
General notes

Clone OTI1A2 (formerly 1A2).
This product was previously labelled as Tristetraprolin

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI1A2
Isotype

IgG2a
Research areas

Western blot - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

All lanes : Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779) at 1/200 dilution

Lane 1 : SVT2 cell extract
Lane 2 : A549 (human lung carcinoma cell line) cell extract
Lane 3 : MDCK (canine kidney cell line) cell extract

Lysates/proteins at 10 µg per lane.

Predicted band size: 34 kDa
Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Tristetraprolin/TTP with ab119779 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
Western blot - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779) at 1/200 dilution + Mouse liver extract at 10 µg

Predicted band size: 34 kDa
Western blot - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

All lanes : Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779) at 1/2000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 34 kDa
Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling Tristetraprolin/TTP with ab119779 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human bladder carcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human breast tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human endometrium tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human lung carcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human pancreas carcinoma tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (ab119779)

Paraffin-embedded human tonsil tissue stained for Tristetraprolin/TTP using ab119779 at 1/150 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com