Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant rat CNTF protein (ab202798)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: HPLC, SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant rat CNTF protein
    See all CNTF proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Human TF-1 cells is less than 30 ng/mL, corresponding to a specific activity of > 3.3 × 104 IU/mg.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P20294

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rat

    • Sequence

      AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLD SVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPT EGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGL FEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM

    • Predicted molecular weight

      23 kDa

    • Amino acids

      2 to 200

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 2% Trehalose, 97% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at

    General Info

    • Alternative names

      • Ciliary neurotrophic factor
      • CNTF
      • CNTF_HUMAN
      • HCNTF

    • Function

      CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

    • Tissue specificity

      Nervous system.

    • Sequence similarities

      Belongs to the CNTF family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (0)

Publishing research using ab202798? Please let us know so that we can cite the reference in this datasheet.

ab202798 has not yet been referenced specifically in any publications.

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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