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Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

Key features and details

  • Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3]
    See all Cytochrome C Oxidase subunit VIc/COX6C primary antibodies

  • Description

    Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, bovine heart, and rat heart. IF/ICC: HeLa cell line

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as Cytochrome C Oxidase subunit VIc

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 50 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg

    Predicted band size: 9 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

    ICC/IF image of ab110267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Overlay histogram showing HepG2 cells stained with ab110267 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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