Anti-VAV2 antibody - C-terminal (ab226955)
Key features and details
- Rabbit polyclonal to VAV2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VAV2 antibody - C-terminal
See all VAV2 primary antibodies -
Description
Rabbit polyclonal to VAV2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Rhesus monkey
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Immunogen
Recombinant fragment within Human VAV2 (C terminal). The exact sequence is proprietary.
Database link: P52735 -
Positive control
- IP: HepG2 whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse olfactory bulb tissue extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-VAV2 antibody - C-terminal (ab226955)
VAV2 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract using 5 µg of ab226955. Western blot was performed from the immunoprecipitate using ab226955.
Lane 1: Control IgG IP in HepG2 whole cell extract.
Lane 2: ab226955 IP in HepG2 whole cell extract.
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Western blot - Anti-VAV2 antibody - C-terminal (ab226955)All lanes : Anti-VAV2 antibody - C-terminal (ab226955) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 101 kDa7.5% SDS-PAGE
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Western blot - Anti-VAV2 antibody - C-terminal (ab226955)Anti-VAV2 antibody - C-terminal (ab226955) at 1/3000 dilution + Mouse olfactory bulb tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 101 kDa7.5% SDS-PAGE gel.
