Anti-NOB1 antibody (ab224619)
Key features and details
- Rabbit polyclonal to NOB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NOB1 antibody
See all NOB1 primary antibodies -
Description
Rabbit polyclonal to NOB1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment corresponding to Human NOB1 aa 100-250.
Database link: Q9ULX3 -
Positive control
- WB: HeLa whole cell lysate; Mouse brain, kidney and stomach tissue lysates. IHC-P: Human thyroid and placenta tissues. ICC/IF: A549 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NOB1 antibody (ab224619) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse stomach tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Paraffin-embedded human thyroid tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human placenta tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.
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A549 (human lung carcinoma cell line) cells stained for NOB1 (green) using ab224619 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).