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Human Axl ELISA Kit (ab208987)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Axl ELISA Kit
    See all Axl kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.9%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    11 pg/ml

  • Range

    15.6 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 90 87% - 95%
    Urine 108 102% - 115%
    Serum 110 109% - 111%
    Cell culture media 99 97% - 100%
    Hep Plasma 105 105% - 106%
    EDTA Plasma 108 107% - 109%
    Cit plasma 107 105% - 110%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Goat, Cow, Pig

  • Product overview

    Human Axl ELISA Kit (ab208987) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Axl protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Axl with 11 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Axl is a transmembrane receptor tyrosine kinase on the plasma membrane. The growth factor GAS6 is a ligand for Axl and regulates many physiological processes including cell survival, cell proliferation, migration and differentiation. The antibodies used in this kit were raised against a portion of the extracellular domain of Axl.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Axl ELISA Kit (ab208987)
    Other - Human Axl ELISA Kit (ab208987)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human Axl standard curve.
    Example of human Axl standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Axl in human serum, and plasma samples.
    Interpolated concentrations of native Axl in human serum, and plasma samples.

    The concentrations of Axl were measured in duplicates, interpolated from the Axl standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 2%, plasma (EDTA) 0.5% and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 66 ng/mL in serum, 56 ng/mL in plasma (citrate) and 66 ng/mL in plasma (EDTA) and 64 ng/mL in plasma (heparin).

  • Interpolated concentrations of native Axl in human urine and milk samples.
    Interpolated concentrations of native Axl in human urine and milk samples.

    The concentrations of Axl were measured in duplicates, interpolated from the Axl standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 0.25% and milk 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 271 ng/mL in urine and 2.38 ng/mL in milk.

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 58.1 ng/mL with a range of 37.8 – 89.9 ng/mL.

  • Sandwich ELISA - Human Axl ELISA Kit (ab208987)
    Sandwich ELISA - Human Axl ELISA Kit (ab208987)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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