Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5648] to BST2/Tetherin - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free
    See all BST2/Tetherin primary antibodies

  • Description

    Rabbit monoclonal [EPR5648] to BST2/Tetherin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and A431 whole cell lysates.

  • General notes

    ab232454 is the carrier-free version of ab134061 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232454 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BST2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
    Western blot - Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
    All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : BST2 knockout A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    ab134061 was shown to react with BST2 in A431 wild-type cells in western blot. Loss of signal was observed when BST2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
    Western blot - Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
    Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134061).

  • Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
    Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)

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