Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLIP170 antibody [mAbcam61830] (ab61830)

Key features and details

  • Mouse monoclonal [mAbcam61830] to CLIP170
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CLIP170 antibody [mAbcam61830]
    See all CLIP170 primary antibodies

  • Description

    Mouse monoclonal [mAbcam61830] to CLIP170

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CLIP170 aa 1100 to the C-terminus.
    (Peptide available as ab96632)

  • Positive control

    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Anti-CLIP170 antibody [mAbcam61830] (ab61830) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 161 kDa
    Observed band size: 161 kDa
    Additional bands at: 190 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)

    ICC/IF image of ab61830 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61830 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa, HepG2, and MCF-7 cell lines in PFA at 10ug/ml

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    IHC image of Ab61830 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab61830, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Flow Cytometry - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Flow Cytometry - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Overlay histogram showing HeLa cells stained with ab61830 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61830, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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