Mouse Apolipoprotein CIII Matched Antibody Pair Kit (ab219538)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse Apolipoprotein CIII Matched Antibody Pair Kit
See all Apolipoprotein CIII kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
16.9 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Apolipoprotein CIII Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Apolipoprotein CIII.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab217777. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Apolipoprotein CIII Capture Antibody 2 x 50µg 1 x 50µg Mouse Apolipoprotein CIII Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Apolipoprotein CIII Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
Tissue specificity
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
Involvement in disease
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
Sequence similarities
Belongs to the apolipoprotein C3 family. -
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APOC3
- APO C3
- Apo CIII
see all -
Database links
- Entrez Gene: 11814 Mouse
- SwissProt: P33622 Mouse
- Unigene: 390161 Mouse
Images
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Mouse Apolipoprotein CIII Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Apolipoprotein CIII Matched Antibody Pair Kit (ab219538)To learn more about the advantages of recombinant antibodies see here.
