Mouse Apolipoprotein CIII ELISA Kit (ab217777)
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Other - Mouse Apolipoprotein CIII ELISA Kit (ab217777)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Apolipoprotein CIII standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Apolipoprotein CIII standard curve in 1X Cell Extraction buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Apolipoprotein CIII in mouse serum and plasma samples.The concentrations of Apolipoprotein CIII were measured in duplicate, interpolated from the Apolipoprotein CIII standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20,000, plasma (citrate) 1:20,000, plasma (EDTA) 1:20,000, and plasma (heparin) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein CIII concentration was determined to be 102.29 µg/mL in neat serum, 94.12 µg/mL in neat plasma (citrate), 111.95 µg/mL in neat plasma (EDTA), and 97.91 µg/mL in neat plasma (heparin).
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Interpolated concentrations of native Apolipoprotein CIII in mouse supernatant samples.The concentrations of Apolipoprotein CIII were measured in duplicate, interpolated from the Apolipoprotein CIII standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 50% and liver supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein CIII concentration was determined to be 13.17 ng/mL in neat lung supernatant and 36.64 ng/mL in neat liver supernatant.
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Interpolated concentrations of native Apolipoprotein CIII in mouse lung and liver tissue extract samples based on a 125 µg/mL extract load.The concentrations of Apolipoprotein CIII were measured in duplicate and interpolated from the Apolipoprotein CIII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein CIII concentration was determined to be 7.06 ng/mL in lung extract and 16.20 ng/mL in liver extract.
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Sandwich ELISA - Mouse Apolipoprotein CIII ELISA Kit (ab217777)To learn more about the advantages of recombinant antibodies see here.
