Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MDA5 antibody [EPR6743] (ab126630)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6743] to MDA5
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MDA5 antibody [EPR6743]
    See all MDA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR6743] to MDA5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human MDA5. (Q9BYX4)

  • Positive control

    • THP1 treated with LPS cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)
    Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MDA5 knockout HAP1 cell lysate (20 µg)
    Lane 3: THP cell lysate with 100 nM PAM for 6 h (20 µg)
    Lane 4: THP cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab126630 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab126630 was shown to specifically react with MDA5 when MDA5 knockout samples were used. Wild-type and MDA5 knockout samples were subjected to SDS-PAGE. ab126630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)
    Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)
    Anti-MDA5 antibody [EPR6743] (ab126630) at 1/1000 dilution + THP1 treated with LPS cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 117 kDa

  • Anti-MDA5 antibody [EPR6743] (ab126630)
    Anti-MDA5 antibody [EPR6743] (ab126630)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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