Anti-MDA5 antibody (ab79055)
Key features and details
- Rabbit polyclonal to MDA5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MDA5 antibody
See all MDA5 primary antibodies -
Description
Rabbit polyclonal to MDA5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
16 amino acid peptide from near the carboxy terminus of human MDA5.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (ab79055)
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse kidney using 5 µg/ml ab79055
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Western blot - Anti-MDA5 antibody (ab79055)Lane 1 : Anti-MDA5 antibody (ab79055) at 1 µg/ml
Lane 2 : Anti-MDA5 antibody (ab79055) at 2 µg/ml
Lane 3 : Anti-MDA5 antibody (ab79055) at 4 µg/ml
All lanes : Daudi cell lysate
Predicted band size: 117 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-MDA5 antibody (ab79055)ICC/IF image of ab79055 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79055, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (ab79055)Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse pancreas using 5 µg/ml ab79055
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (ab79055)Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse small intestine using 5 µg/ml ab79055
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (ab79055)Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed human pancreas using 5 µg/ml ab79055
