Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)
![Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)](https://www.abcam.com/ps/products/219/ab219952/Images/ab219952-403311-anti-mda5-antibody-epr6743-bsa-and-azide-free-western-blot.jpg "Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6743] to MDA5 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MDA5 antibody [EPR6743] - BSA and Azide free
See all MDA5 primary antibodies -
Description
Rabbit monoclonal [EPR6743] to MDA5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab219952 is the carrier-free version of ab126630 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab219952 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6743 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)
This data was developed using ab126630, the same antibody clone in a different buffer formulation.
Lane 1 Wild-type HAP1 cell lysate (20 µg)
Lane 2 MDA5 knockout HAP1 cell lysate (20 µg)
Lane 3 THP cell lysate with 100 nM PAM for 6 h (20 µg)
Lane 4 THP cell lysate (20 µg)
Lanes 1 - 4 Merged signal (red and green). Green - ab126630 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab126630 was shown to specifically react with MDA5 when MDA5 knockout samples were used. Wild-type and MDA5 knockout samples were subjected to SDS-PAGE. ab126630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)](https://www.abcam.com/ps/products/219/ab219952/Images/ab219952-399164-anti-mda5-antibody-epr6743-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952) -
![Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)](https://www.abcam.com/ps/products/219/ab219952/Images/ab219952-3-benefits-of-recombinant-antibodies.png)
Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)
